AI Article Synopsis
- The study aimed to assess how different irrigation protocols affect the structure of root dentin using advanced microscopy techniques (SEM and TEM).
- Thirty-nine bovine incisors were divided into 13 groups, each receiving a specific irrigation solution, including controls (distilled water) and various combinations with sodium hypochlorite (NaOCl), EDTA, and other agents.
- Results indicated that NaOCl, particularly when used with PUI or EDTA, significantly altered the dentin structure by thinning collagen fibrils and causing erosion, highlighting the importance of selecting appropriate irrigation solutions to maintain dentin quality during treatment.
Article Abstract
Objectives: The aim of this study was to evaluate the effect of different irrigation protocols on the root dentin structure using scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
Materials And Methods: Thirty-nine lower bovine incisors were hemisected longitudinally and randomly divided into 13 groups (n = 3). After the root halves were reassembled, it was applied a specific irrigation protocol for each group, as following: G1, distilled water (control); G2, 0.9% saline; G3, saline + 17% EDTA; G4, saline + PUI; G5, saline + PUI + EDTA; G6 to G9 received the same protocol as above replacing 0.9% saline by 2.5% NaOCl; and G10 to G13 by 2% CHX. One-half of each sample was prepared and evaluated using SEM and the other one by TEM observations.
Results: TEM descriptive analysis showed modifications in dentin organic ultrastructure, characterized by the thinning of dentin collagen fibrils, caused by NaOCl, enhanced by EDTA and/or PUI. SEM analysis showed that NaOCl with PUI caused significantly larger erosion of the peritubular dentin than in all the other groups (P < 0.05), followed by NaOCl + EDTA and NaOCl + EDTA + PUI.
Conclusions: NaOCl caused ultrastructural alterations in the dentin collagen, and enhanced by EDTA and/or PUI, promoted peritubular and intertubular erosion.
Clinical Relevance: The effect of irrigating solutions on dentin ultrastructure was still unclear. The acknowledgment about the kind of solution, concentrations, application time, and sequence of use was important to achieve the right sanitization without jeopardizing the dentin ultrastructure quality.
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