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HMGB1 Promotes Intraoral Palatal Wound Healing through RAGE-Dependent Mechanisms. | LitMetric

AI Article Synopsis

  • - High mobility group box 1 (HMGB1) plays a crucial role in tissue healing after injury, particularly in the regeneration of epithelial and connective tissue during palatal wound healing.
  • - In experiments with both heterozygous HMGB1 and wild-type mice, differences in tissue recovery were observed, with wild-type mice showing better collagen organization and greater cell proliferation markers (PCNA, NF-κB, VEGF) after injury.
  • - The study indicates that the interaction between HMGB1 and its receptor, RAGE, is essential for promoting cell migration and tissue regeneration in palatal wounds, as blocking RAGE inhibited the beneficial effects of HMGB1.

Article Abstract

High mobility group box 1 (HMGB1) is tightly connected to the process of tissue organization upon tissue injury. Here we show that HMGB1 controls epithelium and connective tissue regeneration both in vivo and in vitro during palatal wound healing. Heterozygous HMGB1 () mice and Wild-type (WT) mice were subjected to palatal injury. Maxillary tissues were stained with Mallory Azan or immunostained with anti-HMGB1, anti-proliferating cell nuclear antigen (PCNA), anti-nuclear factor-κB (NF-κB) p50 and anti-vascular endothelial growth factor (VEGF) antibodies. Palatal gingival explants were cultured with recombinant HMGB1 (rHMGB1) co-treated with siRNA targeting receptor for advanced glycation end products (RAGEs) for cell migration and PCNA expression analysis. Measurement of the wound area showed differences between and WT mice on Day 3 after wounding. Mallory Azan staining showed densely packed of collagen fibers in WT mice, whereas in mice weave-like pattern of low density collagen bundles were present. At three and seven days post-surgery, PCNA, NF-κB p50 and VEGF positive keratinocytes of WT mice were greater than that of mice. Knockdown of RAGE prevents the effect of rHMGB1-induced cell migration and PCNA expression in gingival cell cultures. The data suggest that HMGB1/RAGE axis has crucial roles in palatal wound healing.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133955PMC
http://dx.doi.org/10.3390/ijms17111961DOI Listing

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