Preparation of epigallocatechin gallate-loaded nanoparticles and characterization of their inhibitory effects on growth and .

A variety of approaches have been proposed for overcoming the unpleasant side effects associated with antibiotics treatment of () infections. Research has shown that epigallocatechin-3-gallate (EGCG), a major ingredient in green tea, has antibacterial activity for antiurease activity against . Oral EGCG is not good because of its digestive instability and the fact that it often cannot reach the targeted site of antibacterial activity. To localize EGCG to infection site, this study developed a fucose-chitosan/gelatin nanoparticle to encapsulate EGCG at the target and make direct contact with the region of microorganisms on the gastric epithelium. Analysis of a simulated gastrointestinal medium indicated that the proposed nanocarrier system effectively controls the release of EGCG, which interacts directly with the intercellular space at the site of infection. Meanwhile, results of clearance assays indicated that our prepared fucose-chitosan/gelatin/EGCG nanoparticles had a significantly greater clearance effect and more effectively reduced -associated gastric inflammation in the gastric-infected mouse model than the EGCG solution alone.