AI Article Synopsis

  • The RSC chromatin remodeler creates an irregular nucleosome structure called a remosome, which has 30-40 extra base pairs of DNA compared to standard nucleosomes.
  • Large-scale molecular simulations have helped to outline the potential structure of remosomes and revealed their stability even without the RSC protein present.
  • The study highlights that local kinks in DNA are crucial for accommodating this extra DNA without disrupting the binding between DNA and histones.

Article Abstract

It has been shown experimentally that the action of the RSC chromatin remodeler leads to the formation of an irregular, partially remodeled nucleosome, termed a remosome. The remosome contains an extra 30-40 base pairs of DNA compared to a canonical nucleosome. Large-scale molecular simulations have provided information on the probable structure of remosomes and have explained why they remain stable in the absence of RSC. Here we explain how these simulations were carried out and what the resulting remosome models imply in terms of the mechanism of action of RSC. We notably show that local kinks within DNA are key in explaining how extra DNA can be in added to nucleosomes without unduly disturbing DNA-histone binding.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5214536PMC
http://dx.doi.org/10.1080/19491034.2016.1260800DOI Listing

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