AI Article Synopsis
- MicroRNAs are small, noncoding RNAs that help regulate gene expression after transcription and are important in cancer progression as biomarkers.
- EBV (Epstein-Barr Virus) produces 25 viral precursor microRNAs during its infection stages and also triggers specific cellular oncogenic miRNAs that assist in the survival of infected cells.
- The chapter discusses various techniques currently used to identify and measure the expression of both viral and cellular miRNAs in cells infected by EBV.
Article Abstract
MicroRNAs are small, noncoding RNAs that posttranscriptionally regulate gene expression. The discovery of this relatively new mode of gene regulation as well as studies showing the prognostic value of viral and cellular miRNAs as biomarkers, such as in cancer progression, has stimulated the development of many methods to characterize miRNAs. EBV encodes 25 viral precursor microRNAs within its genome that are expressed during lytic and latent infection. In addition to viral miRNAs, EBV infection induces the expression of specific cellular oncogenic miRNAs, such as miR-155, miR-146a, miR-21, and others, that can contribute to the persistence of latently infected cells. This chapter describes several current techniques used to identify and detect the expression of viral and cellular miRNAs in EBV-infected cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5685176 | PMC |
| http://dx.doi.org/10.1007/978-1-4939-6655-4_9 | DOI Listing |
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