Absolute protein quantification is an essential tool for system biology approaches and elucidation of stoichiometry of multi-protein complexes. In this chapter, a universal protocol for gel free absolute protein quantification in bacterial systems is described, which can be used for sample preparation prior to miscellaneous mass-spectrometry-based quantification workflows like AQUA, Hi3, and emPAI. In addition, a focus has been set to the specific challenges in antibiotic stress research.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-6634-9_17 | DOI Listing |
Publication Analysis
Top Keywords
absolute protein
12
protein quantification
12
sample preparation
8
antibiotic stress
8
preparation mass-spectrometry
4
mass-spectrometry based
4
based absolute
4
quantification
4
quantification antibiotic
4
stress absolute
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!