AI Article Synopsis
- Researchers created a new reverse genetics system for West Nile virus (WNV) using mammalian cells due to instability issues in E. coli.
- They successfully produced infectious WNV by combining specific DNA fragments coding for structural proteins with a replicon.
- The resulting recombinant virus showed similar growth rates and plaque sizes to the original WNV, allowing for better research on WNV infection.
Article Abstract
Reverse genetics systems facilitate investigation of many aspects of the life cycle and pathogenesis of viruses. However, genetic instability in Escherichia coli has hampered development of a reverse genetics system for West Nile virus (WNV). In this study, we developed a novel reverse genetics system for WNV based on homologous recombination in mammalian cells. Introduction of the DNA fragment coding for the WNV structural protein together with a DNA-based replicon resulted in the release of infectious WNV. The growth rate and plaque size of the recombinant virus were almost identical to those of the parent WNV. Furthermore, chimeric WNV was produced by introducing the DNA fragment coding for the structural protein and replicon plasmid derived from various strains. Here, we report development of a novel system that will facilitate research into WNV infection.
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| http://dx.doi.org/10.1016/j.jviromet.2016.11.006 | DOI Listing |
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