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Evaluation of the modified carbapenem inactivation method and sodium mercaptoacetate-combination method for the detection of metallo-β-lactamase production by carbapenemase-producing Enterobacteriaceae. | LitMetric
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Evaluation of the modified carbapenem inactivation method and sodium mercaptoacetate-combination method for the detection of metallo-β-lactamase production by carbapenemase-producing Enterobacteriaceae.

Kageto Yamada Machiko Kashiwa Katsumi Arai Noriyuki Nagano Ryoichi Saito

J Microbiol Methods

Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan.

Published: January 2017

We evaluated the effectiveness of carbapenem inactivation method (CIM) and modified CIM (mCIM). Our results indicated that mCIM with 4h incubation improved sensitivity and specificity for detecting carbapenemase-producing Enterobacteriaceae compared to CIM. Additionally, we developed a sodium mercaptoacetate-combination method (SMA-mCIM) to detect metallo-β-lactamase (MBL) with high sensitivity and specificity.

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http://dx.doi.org/10.1016/j.mimet.2016.11.013DOI Listing

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