AI Article Synopsis
- - The HSV-1 UL2 protein plays a key role in viral infection but its cellular location and transport mechanisms were previously unclear.
- - Researchers used fluorescent microscopy to observe that the UL2 protein accumulates in the nucleus of live cells without needing other HSV-1 proteins present.
- - The study identified that multiple cellular transport pathways, involving several transport receptors, assist in the nuclear transport of UL2, enhancing our understanding of its role in HSV-1 infection.
Article Abstract
As a crucial protein, the herpes simplex virus 1 (HSV-1) UL2 protein has been shown to take part in various stages of viral infection, nonetheless, its exact subcellular localization and transport molecular determinants are not well known thus far. In the present study, by using live cells fluorescent microscopy assay, UL2 tagged with enhanced yellow fluorescent protein was transiently expressed in live cells and showed a completely nuclear accumulation without the presence of other HSV-1 proteins. Moreover, the nuclear transport of UL2 was characterized to be assisted by multiple transport pathways through Ran-, importin α1-, α5-, α7-, β1- and transportin-1 cellular transport receptors. Consequently, these results will improve understanding of UL2-mediated biological functions in HSV-1 infection cycles.
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| http://dx.doi.org/10.1515/hsz-2016-0268 | DOI Listing |
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