AI Article Synopsis
- Plant cell wall glycans are essential for plant growth and are a valuable source of eco-friendly biomass.
- This study focuses on mapping the specific region that the monoclonal antibody (mAb) LM5 binds to, which is linked to linear beta-1,4 galactan.
- The research employs synthetic galactan analogues and various analytical techniques like carbohydrate microarrays and ELISA to investigate how the antibody interacts with the carbohydrates.
Article Abstract
Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies (mAbs) for localization and visualization of glycans, using e.g. immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mAb LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogues of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.
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| http://dx.doi.org/10.1016/j.carres.2016.10.012 | DOI Listing |
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