Oxidatively Locked [Co₂L₃] Cylinders Derived from Bis(bidentate) 2-Pyridyl-1,2,3-triazole "Click" Ligands: Synthesis, Stability, and Antimicrobial Studies.

A small family of [Co₂()₃] cylinders was synthesised from bis(bidentate) 2-pyridyl-1,2,3-triazole "click" ligands () through an "assembly-followed-by-oxidation" method. The cylinders were characterised using ¹H, C, and DOSY NMR, IR, and UV-Vis spectroscopies, along with electrospray ionisation mass spectrometry (ESMS). Stability studies were conducted in dimethyl sulfoxide (DMSO) and D₂O. In contrast to similar, previously studied, [Fe₂()₃] helicates the more kinetically inert [Co₂()₃] systems proved stable (over a period of days) when exposed to DMSO and were even more stable in D₂O. The triply stranded [Co₂()₃] systems and the corresponding "free" ligands were tested for antimicrobial activity in vitro against both Gram-positive () and Gram-negative () microorganisms. Agar-based disk diffusion and Mueller-Hinton broth micro-dilution assays showed that the [Co₂()₃] cylinders were not active against either strain of bacteria. It is presumed that a high charge of the [Co₂()₃] cylinders is preventing them from crossing the bacterial cell membranes, rendering the compounds biologically inactive.