A study was conducted in order to evaluate the contamination by spp. of meat from animals slaughtered because they had resulted positive for brucellosis at some time during their life. After slaughter and before delivery to market outlets, swab samples were taken from 307 carcasses of infected animals: 40 cattle, 60 sheep and 207 goats. The swabs were subsequently analysed by means of polymerase chain reaction (PCR) tests. In addition, bacteriological tests were carried out on the lymph nodes and internal organs of the same animals. spp. was detected by means of PCR in 25/307 carcasses (8%): 1 bovine (2.5%), 9 sheep (15%) and 15 goats (7.2%) and was isolated by means of a cultural method in 136/307 carcasses (44%). Moreover, additional analysis, performed on lymph nodes from the same carcasses that had proved positive by PCR, allowed highlighting type 3 in the bovine carcass and type 3 in the sheep and goat carcasses. The study shows that cattle, sheep and goats meat of animals slaughtered because they had tested positive for brucellosis may be contaminated by spp. As this could constitute a real risk of transmission to both butchery personnel and consumers, the meat of animals infected by spp. should be analysed before being marketed. In this respect, PCR technique performed on swabs proved to be more useful, practical and faster than the traditional bacteriological method.
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http://dx.doi.org/10.4081/ijfs.2016.5913 | DOI Listing |
BMC Vet Res
January 2025
Theriogenology Department, Faculty of Veterinary Medicine, New-Valley University, New Valley, 725211, Egypt.
Background: Saidi sheep are one of the most important farm animals in Upper Egypt, particularly in the Assiut governorate. Since they can provide meat, milk, fiber, and skins from low-quality roughages, sheep are among the most economically valuable animals bred for food in Egypt. Regarding breeding, relatively little is known about the Saidi breed.
View Article and Find Full Text PDFAMB Express
January 2025
Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams University, Hadayek Shoubra, P.O. Box 68, Cairo, 11241, Egypt.
Valorization of poultry waste is a significant challenge addressed in this study, which aimed to produce cost-effective and sustainable peptones from poultry waste. The isolation process yielded the highly potent proteolytic B.subtilis isolate P6, identified through 16S rRNA gene sequencing to share 94% similarity with the B.
View Article and Find Full Text PDFZhongguo Zhong Yao Za Zhi
December 2024
Experimental Research Center, China Academy of Chinese Medical Sciences Beijing 100700, China.
Calcined oyster is a commonly used shellfish traditional Chinese medicine in clinical practice in China. During the processing of oysters, their microscopic characteristics are destroyed, and open-fire calcination can damage the DNA of oysters, making it difficult to identify the primary source. The establishment of a specific polymerase chain reaction(PCR) method for the identification of calcined oysters can provide a guarantee for the safety and clinical efficacy of the medicine and its processed products.
View Article and Find Full Text PDFPLoS One
January 2025
Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
Lumpy skin disease (LSD) is an emerging, highly contagious transboundary disease of bovines caused by the Lumpy skin disease virus (LSDV), responsible for substantial economic losses to the dairy, meat, and leather industries in Pakistan as well as various countries around the world. Epidemiological information on LSD is scarce in Punjab, Pakistan. Therefore, a molecular epidemiological study was conducted in two agro-ecologically diverse districts (Bhakkar and Jhang) of Punjab, Pakistan.
View Article and Find Full Text PDFVet Med Sci
January 2025
Department of Animal Breeding and Husbandry, Istanbul University-Cerrahpasa, Istanbul, Türkiye.
Background: Obtaining information about the growth rates of animals' organs and tissues can help understand their meat production potential and determine the ideal slaughter weight (SW).
Objectives: This study aimed to determine the effects of production system and SW on the allometric growth of the non-carcass components, carcass cuts, and hind limb tissues of Kivircik lambs.
Methods: A total of 54 single-born male lambs were randomly allocated into production systems (concentrate- and pasture-based) and SW groups: 25-26 kg (LOW), 30-31 kg (MEDIUM), and 35-36 kg (HIGH).
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