AI Article Synopsis
- Iron (Fe) and manganese (Mn) are crucial for plant growth, and in Arabidopsis thaliana, their uptake is primarily managed by high affinity transporters IRT1 and NRAMP1.
- Mutant plants lacking both transporters displayed severe Fe deficiency symptoms, even when Fe was present in adequate amounts, indicating the importance of these transporters for Fe uptake.
- The study revealed that NRAMP1 is essential for Fe transport and works alongside IRT1, providing the first evidence of a low affinity Fe uptake system in plants, while also suggesting a role in Mn uptake.
Article Abstract
Iron (Fe) and manganese (Mn) are essential metals which, when scarce in the growth medium, are respectively taken up by the root high affinity transporters IRT1 and NRAMP1 in Arabidopsis thaliana. The molecular bases for low affinity transport however remained unknown. Since IRT1 and NRAMP1 have a broad range of substrates among metals, we tested the hypothesis that they might be functionally redundant by generating nramp1 irt1 double mutants. These plants showed extreme Fe-deficiency symptoms despite optimal provision of the metal. Their phenotype, which includes low Fe and Mn contents and a defect of Fe entry into root cells as revealed by Fe staining, is rescued by high Fe supply. Using a promoter swap-based strategy, we showed that root endodermis retains the ability to carry out high affinity Fe transport and furthermore might be important to high-affinity Mn uptake. We concluded that NRAMP1 plays a pivotal role in Fe transport by cooperating with IRT1 to take up Fe in roots under replete conditions, thus providing the first evidence for a low affinity Fe uptake system in plants.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5110964 | PMC |
| http://dx.doi.org/10.1038/srep37222 | DOI Listing |
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