Crystal structure analysis of a hypothetical protein (MJ0366) from Methanocaldococcus jannaschii revealed a novel topological arrangement of the knot fold. | LitMetric
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, 600025, India. Electronic address:
Published: January 2017
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Article Abstract
The crystal structure of a hypothetical protein MJ0366, derived from Methanocaldococcus jannaschii was solved at 1.9 Å resolution using synchrotron radiation. MJ0366 was crystallized as a monomer and has knot structural arrangement. Intriguingly, the solved structure consists of novel 'KNOT' fold conformation. The 3 trefoil knot was observed in the structure. The N-terminal and C-terminal ends did not participate in knot formation.
The ability to rapidly charge batteries is crucial for widespread electrification across a number of key sectors, including transportation, grid storage, and portable electronics. Nevertheless, conventional Li-ion batteries with organic liquid electrolytes face significant technical challenges in achieving rapid charging rates without sacrificing electrochemical efficiency and safety. Solid-state batteries (SSBs) offer intrinsic stability and safety over their liquid counterparts, which can potentially bring exciting opportunities for fast charging applications.
Dion-Jacobson (DJ) perovskites are promising alternatives for Ruddlesden-Popper (RP) perovskites to fabricate blue perovskite light-emitting diodes (PeLEDs) due to their favorable structural and charge properties. However, the relatively weak hydrogen bond between the bridging diammonium group and perovskite poses huge challenges for regulating crystallization and defect density, leading to an undesirable film quality and device performance. Herein, we report the successful optimization of DJ perovskite films by introducing a new type of cesium octafluoroadipate (CsOFAA) precursor, which could strongly anchor the perovskite through coordination bonds and halogen-halogen bonds.
In the present study, an algae-containing octocoral, Junceella fragilis, was subjected to chemical screening. The analysis resulted in the extraction of six polyacetoxybriaranes: a new compound, identified as fragilide Z (1), alongside previously identified analogs, which included 12-epi-fragilide G (2), fragilide P (3), junceellolide D (4), junceellonoid A (5), and juncin ZI (6). The structures of compounds 2-6 were investigated through single-crystal X-ray diffraction analysis, whereas that of 1 was examined through two-dimensional nuclear magnetic resonance analysis.
To explore the effects of the components in the raw materials and by-products of co-pyrolysis on the physicochemical properties of biochar, rice husk （RH, which has a high percentage of lignin and a low content of N） and sawdust （SD, which has a high percentage of both cellulose and N） were used as typical raw materials to prepare co-pyrolysis biochar. The benzene vapor adsorption performance of the obtained biochar was then tested on a fixed-bed device. At the same time, the by-product components generated during pyrolysis were analyzed using thermogravimetric （TG）, scanning electron microscopy （SEM）, and gas chromatography-mass spectrometry （GC-MS）.
Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China.
X-ray crystallography is a fundamental technique that provides atomic-level insights into RNA structures. However, obtaining crystals of RNA structures diffracting to high resolution is challenging. We introduce a simple strategy to enhance the resolution limit of RNA crystals by the selective substitution of Watson-Crick pairs by GU pairs within RNA sequences.
