Premise Of The Study: Microsatellite markers were developed for the rare sedge (Cyperaceae) to assess genetic variation and its spatial structuring.
Methods And Results: We conducted shotgun sequencing on an Illumina MiSeq and produced 6,215,872 sequence reads. The QDD pipeline was used to design 60 primer pairs that were screened using PCR. We developed 17 loci, of which 12 loci were identified that were polymorphic, amplified reliably, and could be consistently scored. We then screened these loci for variation in individuals from three populations. The number of alleles observed for these 12 loci across the three populations ranged from nine to 19 and expected heterozygosity ranged from 0.41 to 0.89.
Conclusions: These markers will enable the quantification of the potential impact of mining on genetic variation within and establish a baseline for future management of genetic variation of the rare sedge.
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http://dx.doi.org/10.3732/apps.1600083 | DOI Listing |
New Phytol
December 2024
Department of Biology, University of Kentucky, Lexington, KY, 40506, USA.
The effects of single chromosome number change-dysploidy - mediating diversification remain poorly understood. Dysploidy modifies recombination rates, linkage, or reproductive isolation, especially for one-fifth of all eukaryote lineages with holocentric chromosomes. Dysploidy effects on diversification have not been estimated because modeling chromosome numbers linked to diversification with heterogeneity along phylogenies is quantitatively challenging.
View Article and Find Full Text PDFSci Total Environ
December 2024
Systems Ecology, Department of Ecological Science, Faculty of Earth and Life Sciences, VU University, De Boelelaan 1085, 1081 HV Amsterdam, the Netherlands.
Moderate grazing can sustain high species diversity and productivity. However, nitrogen enrichment often reduces species richness while promoting primary productivity, which contradicts the traditional understanding of the positive effect of plant diversity on productivity. Whether the responses of diversity and productivity to N enrichment on a long-term scale conform to those on short-term scale.
View Article and Find Full Text PDFBiodivers Data J
March 2023
Real Jardín Botánico (RJB), CSIC, Madrid, Spain Real Jardín Botánico (RJB), CSIC Madrid Spain.
Background: Knowledge of L. (true sedges) and L. (St.
View Article and Find Full Text PDFSci Total Environ
February 2024
University of Lodz, Faculty of Biology and Environmental Protection, Department of Biogeography, Paleoecology and Nature Conservation, 1/3 Banacha Str., 90-237 Lodz, Poland. Electronic address:
Protection and restoration of the CaCO depositing alkaline fens require an in-depth understanding of these unique and declining ecosystems. The present study investigates the development of the formerly heavy tufa depositing alkaline fen in East-Central Europe after CaCO precipitation markedly declined ca. 5400 cal yr BP.
View Article and Find Full Text PDFBraz J Biol
November 2022
PMAS Arid Agriculture University, Department of Botany, Rawalpindi, Pakistan.
Here, we investigated the relationship between Plant communities and the environment from the moist temperate vegetation of Lakoo mountainous forest District Swat. We sampled data from 162 sampling units (Quadrates) using 1x1m2 for herbs 5x5m2 and shrubs, while 10 x10m2 for trees, systematically considering six elevation gradients between the altitudinal from 1970m to 3095m. We performed statistical analysis like Canonical correspondence analysis (CCA) and TWINSPAN (two-way indicator species analysis) for ecological assessment and clustering of plant communities.
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