The precise transportation of fluorescent probes to the designated location in living cells is still a challenge. Here, we present a new addition to nanopipettes as a powerful tool to deliver fluorescent molecules to a given place in a single cell by electroosmotic flow, indicating favorable potential for further application in single-cell imaging.
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http://dx.doi.org/10.1039/c6cc08125h | DOI Listing |
ACS Nano
December 2024
Aix Marseille Univ, INSERM, SSA, MCT, 27 Bd Jean Moulin, Marseille 13005, France.
Bacterial resistance is a major public health challenge. In Gram-negative bacteria, the synergy between multidrug efflux pumps and outer membrane impermeability determines the intracellular concentration of antibiotics. Consequently, it also dictates antibiotic activity on their respective targets.
View Article and Find Full Text PDFBr J Dermatol
December 2024
Department of Dermatology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
Background: The tumour microenvironment significantly influences the clinical response of patients to therapeutic immune checkpoint inhibition (ICI), but a comprehensive understanding of the underlying immune-regulatory proteome is still lacking.
Objectives: To decipher targetable biologic processes that determine tumour-infiltrating lymphocytes (TiLs) as a cellular equivalent of clinical response to ICI.
Methods: We mapped the spatial distribution of proteins in TiL-enriched vs.
J Cancer Res Clin Oncol
December 2024
Moscow Clinical Scientific Center N.A. A.S. Loginov, Moscow, 111123, Russia.
Purpose: Determining the primary origin of non-organ-confined neuroendocrine tumors (NETs) for accurate diagnosis and management. Neuroendocrine tumors are rare neoplasms with diverse clinical behaviors. Determining their primary origin remains challenging in cases of non-organ-confined NETs.
View Article and Find Full Text PDFTalanta
December 2024
Université de Lorraine, CNRS, LIEC, F-54000, Nancy, France.
There is a growing interest in the development of methods for the detection of nanoparticle (NP) toxicity to living organisms based on the analysis of relevant multidimensional data sets. In particular the detection of preliminary signs of NPs toxicity effects would benefit from the selection of data featuring NPs-induced alterations of biological barriers. Accordingly, we present an original Topological Data Analysis (TDA) of the nanomechanical properties of Escherichia coli cell surface, evaluated by multiparametric Atomic Force Microscopy (AFM) after exposure of the cells to increasing concentrations of titanium dioxide nanoparticles (TiONPs).
View Article and Find Full Text PDFSci Data
December 2024
Department of Pathology and Laboratory Medicine, Alpert Medical School, Brown University, Providence, RI, 02912, USA.
In the past several years, a few cervical Pap smear datasets have been published for use in clinical training. However, most publicly available datasets consist of pre-segmented single cell images, contain on-image annotations that must be manually edited out, or are prepared using the conventional Pap smear method. Multicellular liquid Pap image datasets are a more accurate reflection of current cervical screening techniques.
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