Specific and sensitive radioimmunoassay (RIA) systems for human IL-1 alpha and IL-1 beta, using sheep polyclonal antisera, have been developed. The assays, which do not require prior extraction of IL-1, have been used to quantify IL-1 alpha and IL-1 beta in synovial fluid and plasma and to quantify intracellular and extracellular IL-1 alpha and IL-1 beta produced by human monocytes stimulated with endotoxin.
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| http://dx.doi.org/10.1016/0022-1759(89)90212-3 | DOI Listing |
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