AI Article Synopsis
- A bioluminescence test (BT) has been developed to quickly measure lipid oxidation using a specific strain of bacteria (Vibrio harveyi M42) that lights up in the presence of certain aldehydes.
- The method involves treating oils or fats with a CO2+ ion in an alcohol solution at a basic pH, breaking down harmful hydroperoxides into long-chain aldehydes, which the bacteria use to emit light.
- The test proved effective for corn, soybean, and safflower oils, showing results that closely match those of the traditional peroxide value assay used for measuring lipid oxidation.
Article Abstract
A simple, rapid bioluminescence test (BT) for the determination of lipid oxidation is described. The test utilizes an aldehyde-requiring dark mutant of Vibrio harveyi (M42) that emits light in the presence of long chain (C8-C16) aliphatic aldehydes. The procedure consists of treating the oil or fat with CO2+ ion in ethanolic medium at alkaline pH. This treatment facilitates the decomposition of the hydroperoxides into long-chain aldehydes, part of which is used by the bacteria to produce light. The test was evaluated with corn, soybean and safflower oils, and shows excellent correlation with the commonly used peroxide value assay.
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| http://dx.doi.org/10.1002/bio.1170030306 | DOI Listing |
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