The social amoeba is a well-established model organism to study the interaction between bacteria and phagocytes. In contrast, research using as a host model for fungi is rare. We describe a comprehensive study, which uses as a host model system to investigate the interaction with apathogenic () and pathogenic ( sp.) yeast. We show that can be co-cultivated with yeasts on solid media, offering a convenient test to study the interaction between fungi and phagocytes. We demonstrate that a number of mutants increase (, , ) or decrease () the ability of the amoebae to predate yeast cells. On the yeast side, growth characteristics, reduced phagocytosis rate, as well as known virulence factors of () contribute to the resistance of yeast cells against predation by the amoebae. Investigating haploid strains, we suggest using the amoebae plate test for screening purposes after random mutagenesis. Finally, we discuss the potential of our adapted amoebae plate test to use for risk assessment of yeast strains.
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| http://dx.doi.org/10.3389/fmicb.2016.01665 | DOI Listing |
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