Polymorphisms of folate metabolism genes in patients with cirrhosis and hepatocellular carcinoma.

Aim: To evaluated the association of the risk factors and polymorphisms in , , and genes.

Methods: Patients with cirrhosis ( = 116), hepatocellular carcinoma (HCC) ( = 71) and controls ( = 356) were included. Polymerase chain reaction followed by enzymatic digestion and allelic discrimination technique real-time PCR techniques were used for analysis. MINITAB-14.0 and SNPstats were utilized for statistical analysis.

Results: Showed that age ≥ 46 years (OR = 10.31; 95%CI: 5.66-18.76; < 0.001) and smoking (OR = 0.47; 95%CI: 0.28-0.78; = 0.003) were associated with cirrhosis. Age ≥ 46 years (OR = 16.36; 95%CI: 6.68-40.05; < 0.001) and alcohol habit (OR = 2.01; 95%CI: 1.03-3.89; = 0.039) were associated with HCC. in codominant model (OR = 3.37; 95%CI: 1.52-7.50; = 0.014), recessive model (OR = 3.04; 95%CI: 1.43-6.47; = 0.0051) and additive model (OR = 1.71; 95%CI: 1.16-2.52; = 0.0072) was associated with HCC, as well as in the additive model (OR = 1.68; 95%CI: 1.01-2.77; = 0.047), and in the codominant model (OR = 3.26; 95%CI: 1.54-6.87; < 0.001), dominant model (OR = 2.55; 95%CI: 1.24-5.25; = 0.007) and overdominant model (OR = 3.05; 95%CI: 1.66-5.62; < 0.001). in the additive model (OR = 1.54; 95%CI: 1.02-2.33; = 0.042) and smokers who presented at least one polymorphic allele for (OR = 1.71; 95%CI: 0.77-3.82; = 0.0051) showed increased risk for cirrhosis. There was no association between clinical parameters and polymorphisms.

Conclusion: Age ≥ 46 years, alcohol habit and , and polymorphisms are associated with an increased risk of HCC development; age ≥ 46 years, tobacco habit and the polymorphism are associated with cirrhosis.