This study was aimed at collecting data on presence, dissemination and persistence of in small-scale dairy farms. Six farms (located in Piedmont) were visited three times over 2014: 116 waters (wells and different faucets/pipes) and 117 environmental samples (milking equipments and drains) were collected. Enumeration of was performed, 3-5 colonies/samples were selected for identification via 16SrDNA/ polymerase chain reaction (PCR), and typed by enterobacterial-repetitive-intergenic-consensus (ERIC)-PCR. were detected in 77% of samples. No statistical differences were found among proportions of positives across farms, sample typologies and seasons. Most isolates were (45%), and ERIC-PCR showed 32 persistent types diffused across farms. All in all, spp. represents a challenge, considering its presence over time in water as well as in teat cups, indicating a continuous source of contamination. Moreover, persistency of strains may indicate biofilm-formation and/or sanitisers resistance, therefore emphasising the role of primary production for preventing milk contamination by spp.
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| http://dx.doi.org/10.4081/ijfs.2016.5652 | DOI Listing |
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