AI Article Synopsis
- Super-resolution microscopy usually has great spatial detail but lacks in how fast it can capture images.
- Researchers developed a method called super temporal-resolved microscopy (STReM) that boosts the speed of 2D super-resolution imaging by 20 times.
- This technique involves rotating a phase mask during the imaging process and has been tested successfully, revealing new insights into protein behaviors on surfaces.
Article Abstract
Super-resolution microscopy typically achieves high spatial resolution, but the temporal resolution remains low. We report super temporal-resolved microscopy (STReM) to improve the temporal resolution of 2D super-resolution microscopy by a factor of 20 compared to that of the traditional camera-limited frame rate. This is achieved by rotating a phase mask in the Fourier plane during data acquisition and then recovering the temporal information by fitting the point spread function (PSF) orientations. The feasibility of this technique is verified with both simulated and experimental 2D adsorption/desorption and 2D emitter transport. When STReM is applied to measure protein adsorption at a glass surface, previously unseen dynamics are revealed.
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| http://dx.doi.org/10.1021/acs.jpclett.6b02098 | DOI Listing |
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Article Synopsis
- Super-resolution microscopy usually has great spatial detail but lacks in how fast it can capture images.
- Researchers developed a method called super temporal-resolved microscopy (STReM) that boosts the speed of 2D super-resolution imaging by 20 times.
- This technique involves rotating a phase mask during the imaging process and has been tested successfully, revealing new insights into protein behaviors on surfaces.
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