Development of new restorer lines for CMS system with the use of resynthesized oilseed rape ( L.).

Resynthesized (RS) oilseed rape ( L.) is potentially of great interest for hybrid breeding. However, a major problem with the direct use of RS is the quality of seed oil (high level of erucic acid) and seed meal (high glucosinolate content), which does not comply with double-low quality oilseed rape. Thus, additional developments are needed before RS can be introduced into breeding practice. In this study, RS oilseed rape was obtained through crosses between ssp. var. and ssp. var. . RS plant was then crossed with double-low (00) winter oilseed rape lines containing the gene for Ogura cytoplasmic male sterility (CMS ) system. Populations of doubled haploids (DH) were developed from these F hybrids using the microspore culture method. The seeds of semi-RS DH lines were analyzed for erucic acid and glucosinolate content. Among the populations of semi-RS DHs four 00-quality lines with the gene were selected. Using 344 AFLP markers to estimate genetic relatedness, we showed that the RS lines and semi-RS lines formed clusters that were clearly distinct from 96 winter oilseed rape parental lines of F hybrids.