Objectives: In clinical laboratories it is necessary to know for how long the analytes are stable in the samples with specific storage conditions. Our laboratory has implemented the new Aptio Automation System (AAS) (Siemens Healthcare Diagnostics) where the analyzed samples are stored in a refrigerated storage module (RSM) after being sealed. The aim of the study was to evaluate the stability of serum samples with the AAS and comparing the results with a previous study using a conventional refrigerated system.
Design And Methods: Serum samples from a total of 50 patients were collected and for each of them 27 biochemical analytes were analyzed. The samples were divided in 5 sets of 10 samples. Each set was re-analyzed at one of the following times: 24, 48, 72, 96 and 120h. Stability was evaluated according to the Total Limit of Change (TLC) criteria, which combine both analytical and biologic variation.
Results: A total of 26 out of 27 analytes were stable at the end of the study according to TLC criteria. Lactate dehydrogenase was not stable at 48h observing a decrease in its concentration until the end of the study. In the previous study (conventional storage system) 9 biochemical analytes were not stable with an increase of their levels due to the evaporation process.
Conclusions: The RSM connected to the AAS improves the stability of serum samples. This system avoids the evaporation process due to the sealing of samples and allows better control of the samples during their storage.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.clinbiochem.2016.10.012 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Vitamin D status and its determinants in German elite athletes.
Eur J Appl Physiol
January 2025
Department of Exercise Physiology and Sports Therapy, Institute of Sports Science, Justus Liebig University Giessen, Kugelberg 62, 35394, Giessen, Germany.
Purpose: This study investigated elite German athletes to (1) assess their serum 25(OH)D levels and the prevalence of insufficiency, (2) identify key factors influencing serum 25(OH)D levels, and (3) analyze the association between serum 25(OH)D levels and handgrip strength.
Methods: In this cross-sectional study, a total of 474 athletes (231 female), aged 13-39 years (mean 19.3 years), from ten Olympic disciplines were included.
Epidemiological Study on Humans, Animals and Snails for Schistosomiasis in Two Endemic Municipalities Nearing Elimination in Bohol, the Philippines.
Acta Parasitol
January 2025
World Health Organization Regional Office for Southeast Asia, New Delhi, India.
Purpose: A comprehensive survey was conducted to assess the prevalence of Schistosoma japonicum infection in humans, water buffaloes, and snails in the two endemic municipalities of Talibon and Trinidad in Bohol, Philippines, which are nearing elimination.
Methods And Results: Human stool and blood samples were collected from barangays with snail breeding sites, and results showed higher positivity rates using the rSjTPx-1-ELISA compared to the Kato-Katz technique. Human stool examination for showed a 0.
Unveiling nature's antidiabetic potential in Berberis orthobotrys seeds for alloxan-induced diabetic rats.
Sci Rep
January 2025
Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia.
To illustrate the anti-diabetic properties of Berberis orthobotrys seeds was the aim of the current study. After a series of experiments, two doses of aqueous methanolic extract of the seeds were selected i.e.
View Article and Find Full Text PDFSeroprevalence of bovine viral diarrhea virus infection and its associated risk factors in dairy cattle in and around Sebeta sub city, Ethiopia.
Sci Rep
January 2025
College of Veterinary Medicine and Agriculture, Addis Ababa University, Addis Ababa, Ethiopia.
Bovine viral diarrhea (BVD) is a highly infectious disease affecting cattle, leading to significant economic losses in many countries engaged in cattle production. From February 2022 to October 2022, a study was conducted to determine the seroprevalence and associated risk factors of Bovine Viral Diarrhea Virus (BVDV) in the Sebeta subcity area of Sheger city, Ethiopia. The study utilized a semi-structured questionnaire to gather information on herd management practices from dairy farm owners.
View Article and Find Full Text PDFEffect of radiotherapy exposure on fruquintinib plus sintilimab treatment in refractory microsatellite stable metastatic colorectal cancer: a prospective observation study.
J Immunother Cancer
January 2025
Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Background: Immune checkpoint inhibitors (ICIs) in combination with antiangiogenic drugs have shown promising outcomes in the third-line and subsequent treatments of patients with microsatellite stable metastatic colorectal cancer (MSS-mCRC). Radiotherapy (RT) may enhance the antitumor effect of immunotherapy. However, the effect of RT exposure on patients receiving ICIs and targeted therapy remains unclear.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!