AI Article Synopsis
- The semaphorins and plexins are proteins that influence nerve growth and immune responses, and are now known to affect various developmental and disease processes involving cell adhesion and migration.
- SEMA4D, a member of this family, is crucial for angiogenesis, favoring the movement of endothelial cells that have its receptor, Plexin-B1.
- Some tumors release SEMA4D and other angiogenic proteins to promote blood vessel growth, and this study discusses using specific assays to measure the angiogenic effects of SEMA4D from cells and in solution.
Article Abstract
The semaphorins and plexins comprise a family of cysteine-rich cell surface and secreted proteins originally shown to control nerve growth and the immune response, but that have recently been implicated in a wide variety of developmental and pathological processes that are influenced by cell adhesion and migration. Along those lines, our group and others have found that Semaphorin 4D (SEMA4D) plays an important role in angiogenesis by promoting chemotaxis of endothelial cells, which express its receptor, Plexin-B1. Indeed, some neoplasms produce SEMA4D along with other pro-angiogenic proteins for the purpose of enhancing blood vessel growth into a developing neoplasm. Here we describe the application of in vitro migration and tubulogenesis assays and the directed in vivo angiogenesis assay (DIVAA) in the measurement of the angiogenic potential of cell-derived and soluble SEMA4D.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309191 | PMC |
| http://dx.doi.org/10.1007/978-1-4939-6448-2_31 | DOI Listing |
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