, a root endoparasite of several cereal species, is considered to be non-pathogenic but serves as a vector of various plant viruses belonging to the genera , , and . Specifically, it reduces barley productivity by transmitting the (BaYMV). To date, due to its obligate biotrophic property, no artificial culturing of was reported and its quantification was also technically challenging. Here, we developed a novel and simple method to infect within sterile barley roots in contamination free by preparing nearly pure zoospore inoculum. Such artificial maintenance of was verified based on the presence of various developmental stages in infected barley roots under microscope. In addition, the population of resting spores in host tissue was determined by establishing standard curve between manually counted number of spores and C values of 18S rDNA amplification using quantitative real-time PCR. Furthermore, it was validated that standard curve generated was also applicable to estimate the abundance of in soil environments. In conclusion, the present study would help to generate a system to investigate the etiological causes as well as management of plant diseases caused by and BaYMV in tissue and soil.
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| http://dx.doi.org/10.1007/s12088-016-0608-2 | DOI Listing |
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