AI Article Synopsis
- - In a previous study, researchers used next-generation sequencing to explore the transcriptome and identified potential antimicrobial peptides, focusing on one called scolopendrasin V based on its useful properties.
- - This study examined the effectiveness of scolopendrasin V against bacteria using various assays, including radial diffusion and colony count methods.
- - The mode of action for scolopendrasin V was analyzed and found to involve its binding to specific bacterial membrane components, which could lead to new developments in peptide-based antibiotics.
Article Abstract
In a previous study, we analyzed the transcriptome of using next-generation sequencing technology and identified several antimicrobial peptide candidates. One of the peptides, scolopendrasin V, was selected based on the physicochemical properties of antimicrobial peptides using a bioinformatics strategy. In this study, we assessed the antimicrobial activities of scolopendrasin V using the radial diffusion assay and colony count assay. We also investigated the mode of action of scolopendrasin V using flow cytometry. We found that scolopendrasin V's mechanism of action involved binding to the surface of microorganisms via a specific interaction with lipopolysaccharides, lipoteichoic acid, and peptidoglycans, which are components of the bacterial membrane. These results provide a basis for developing peptide antibiotics.
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| http://dx.doi.org/10.4014/jmb.1609.09057 | DOI Listing |
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