AI Article Synopsis
- Integrin activation plays a key role in regulating leukocyte behavior during inflammation, particularly in rolling, adhesion, and trans-vessel migration.
- This study shows that CD95, when engaged by its ligand CD95L from endothelial cells, mediates important processes like slow rolling and adhesion of neutrophils, and triggers signaling pathways that lead to integrin activation in myeloid cells.
- The research reveals that myeloid cells lacking CD95 are less effective at clearing bacteria in sepsis models, indicating potential therapeutic implications for targeting CD95 in inflammatory diseases.
Article Abstract
Integrin activation is crucial for the regulation of leukocyte rolling, adhesion and trans-vessel migration during inflammation and occurs by engagement of myeloid cells through factors presented by inflamed vessels. However, endothelial-dependent mechanisms of myeloid cell recruitment are not fully understood. Here we show using an autoperfused flow chamber assay of whole blood neutrophils and intravital microscopy of the inflamed cremaster muscle that CD95 mediates leukocyte slow rolling, adhesion and transmigration upon binding of CD95-ligand (CD95L) that is presented by endothelial cells. In myeloid cells, CD95 triggers activation of Syk-Btk/PLCγ2/Rap1 signaling that ultimately leads to integrin activation. Excitingly, CD95-deficient myeloid cells exhibit impaired bacterial clearance in an animal model of sepsis induced by cecal ligation and puncture (CLP). Our data identify the cellular and molecular mechanisms underlying the chemoattractant effect of endothelial cell-derived CD95L in induction of neutrophil recruitment and support the use of therapeutic inhibition of CD95's activity in inflammatory diseases.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098908 | PMC |
| http://dx.doi.org/10.7554/eLife.18542 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nanomembrane on Graphene: Delamination Dynamics and 3D Construction.
ACS Nano
January 2025
Department of Materials Science & International Institute of Intelligent Nanorobots and Nanosystems, State Key Laboratory of Surface Physics, Fudan University, Shanghai 200438, People's Republic of China.
Freestanding nanomembranes fabricated by lift-off technology have been widely utilized in microelectromechanical systems, soft electronics, and microrobotics. However, a conventional chemical etching strategy to eliminate nanomembrane adhesion often restricts material choice and compromises quality. Herein, we propose a nanomembrane-on-graphene strategy that leverages the weak van der Waals adhesion on graphene to achieve scalable and controllable release and 3D construction of nanomembranes.
View Article and Find Full Text PDFBad habits that stick: adhesive tape use practices and beliefs.
Infect Control Hosp Epidemiol
December 2024
Department of Internal Medicine, Baystate Medical Center, Springfield, MA, USA.
Tape rolls are often used for multiple patients despite recommendations by manufacturers for single-patient use. We developed a survey to query Health Care Personnel about their tape use practices and beliefs and uncovered behaviors that put patients at risk for hospital-acquired infections due to tape use.
View Article and Find Full Text PDFBlacking any Material Surface by Amyloid-Fortified Carbon Coating Toward High-Performance Large-Scale Solar Steam Generation System.
Small
December 2024
Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710119, China.
Enhancing the interfacial adhesion between carbon-based coatings and substrates through a simple method remains a challenge, mainly due to the intrinsic chemical inertness of carbon materials. Herein, a carbon nanosphere-based coating utilizing an amyloid-like protein aggregation strategy is developed, involving only the reaction of protein, reductant, and carbon nanospheres in an aqueous solution at room temperature. The resultant coating, enriched in amyloid-like protein structures, features both robust interfacial adhesion and high light absorption (≈98.
View Article and Find Full Text PDFUltra-durable photothermal anti-/de-icing superhydrophobic coating with water droplets freezing from the outside in.
J Colloid Interface Sci
December 2024
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
In low-temperature, high-humidity environments, the condensation of water vapor within microstructures can initiate a detrimental cycle of hydrophobic failure, high-adhesion ice formation, and microstructural degradation, thereby limiting the practical application of superhydrophobic coatings in anti-icing and de-icing technologies. Therefore, enhancing the hydrophobic stability and mechanical durability of these coatings under such conditions is imperative. This study presents a novel approach where rigid FeO nanoparticles are encapsulated within porous diatomaceous earth (DME) and combined with high-adhesion acrylic resin (AR), resulting in a superhydrophobic photothermal coating that possesses both active and passive de-icing capabilities, fabricated through a straightforward one-step spraying technique.
View Article and Find Full Text PDFEffects of rolling on eating quality, starch structure, and water distribution in cooked indica rice dough.
J Sci Food Agric
December 2024
School of Agriculture, Food and Ecosystem Sciences, University of Melbourne, Parkville, Victoria, Australia.
Background: Given the composition of rice and its lack of gluten proteins, rice flour fails to form a cohesive and elastic dough when mixed directly with water. Consequently, many rice products rely on rice sheets (RS) made by rolling cooked rice dough. Limited research exists on how the rolling process impacts the properties and structure of cooked indica rice dough.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!