Background: The rice receptor kinase XA21 confers robust resistance to the bacterial pathogen pv. (). We previously reported that XA21 is cleaved in transgenic plants overexpressing XA21 with a GFP tag (-XA21-GFP) and that the released C-terminal domain is localized to the nucleus. XA21 carries a predicted nuclear localization sequence (NLS) that directs the C-terminal domain to the nucleus in transient assays, whereas alanine substitutions in the NLS disrupt the nuclear localization.
Methods: To determine if the predicted NLS is required for XA21-mediated immunity , we generated transgenic plants overexpressing an XA21 variant carrying the NLS with the same alanine substitutions (-XA21nls-GFP).
Results: XA21nls-GFP plants displayed slightly longer lesion lengths, higher bacterial populations after inoculation and lower levels of reactive oxygen species production compared with the XA21-GFP control plants. However, the XA21nls-GFP plants express lower levels of protein than that observed in XA21-GFP.
Discussion: These results demonstrate that the predicted NLS is not required for XA21-mediated immunity.
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| http://dx.doi.org/10.7717/peerj.2507 | DOI Listing |
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