A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 176

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3122
Function: getPubMedXML

File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

Isolation of a genetically accessible thermophilic xylan degrading bacterium from compost. | LitMetric

Isolation of a genetically accessible thermophilic xylan degrading bacterium from compost.

Biotechnol Biofuels

Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 WE Wageningen, The Netherlands ; Corbion, Arkselsedijk 46, 4206 AC Gorinchem, The Netherlands.

Published: October 2016

AI Article Synopsis

  • The finite nature of global oil resources has led to the search for renewable sources like lactic acid, which is a potential bioplastic precursor, requiring efficient fermentation processes from lignocellulosic materials.
  • Researchers isolated and screened 94 facultative anaerobic (hemi)cellulolytic bacterial strains from compost, ultimately selecting strain T12 for its ability to produce significant amounts of lactic acid while using minimal enzymes.
  • Strain T12 was shown to be genetically accessible and effective in converting both glucose and xylose to lactic acid, positioning it as a promising candidate for developing consolidated bioprocessing techniques to transform biomass into valuable biochemicals.

Article Abstract

Background: Due to the finite nature of global oil resources we are now faced with the challenge of finding renewable resources to produce fuels and chemicals in the future. Lactic acid has great potential as a precursor for the production of bioplastics alternatives to conventional plastics. Efficient lactic acid fermentation from non-food lignocellulosic substrates requires pretreatment and saccharification to generate fermentable sugars. A fermentation process that requires little to no enzyme additions, i.e. consolidated bioprocessing would be preferred and requires lactic acid-producing organisms that have cellulolytic and/or hemicellulolytic activity.

Results: To obtain candidate production strains we have enriched and isolated facultative anaerobic (hemi) cellulolytic bacterial strains from compost samples. By selecting for growth on both cellulose and xylan, 94 strains were isolated. Subsequent screening for lactic acid production was carried out from C6 and C5 sugar fermentations and a selection of the best lactic acid producers was made. The denitrifying T12 was selected for further research and was rendered genetically accessible. In fermentations on a mixture of glucose and xylose, a total of 20.3 g of lactic acid was produced with a yield of 0.94 g product/g sugar consumed. In addition, strain T12 is capable of direct conversion of beech wood xylan to mainly lactic acid in minimal media.

Conclusions: We have demonstrated that T12 is genetically accessible and produces lactic acid as its main fermentation product on glucose, xylose and a mixture thereof. Strain T12 was additionally used for the direct conversion of xylan to lactic acid. The genetic accessibility of the T12 strain provides a solid basis for the development of this strain into a host for consolidated bioprocessing of biomass to lactic acid.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053077PMC
http://dx.doi.org/10.1186/s13068-016-0618-7DOI Listing

Publication Analysis

Top Keywords

lactic acid
36
genetically accessible
12
lactic
10
acid
9
consolidated bioprocessing
8
glucose xylose
8
strain t12
8
direct conversion
8
xylan lactic
8
t12
5

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!