AI Article Synopsis
- Florida's citrus industry is struggling with citrus greening disease, prompting the need for effective treatment methods.
- Penicillin G, along with its metabolites (penillic and penilloic acids), has proven to be one of the most effective antibiotics for this disease.
- A validated ultra-HPLC tandem MS method was developed to detect these compounds at low chemical residue levels, showing high selectivity and recovery rates for analysis.
Article Abstract
Florida citrus depends on a breakthrough in the fight against citrus greening disease. Of the antibiotics used to treat this disease, penicillin G has been one of the most effective. Because orange fruit grown in the state of Florida are mainly used to produce orange juice, we have validated an ultra-HPLC tandem MS method to screen for penicillin G and its metabolites (penillic and penilloic acids) at the chemical residue level after treatment. In this method, three spike levels (0.25, 1, and 20 ng/g) were tested in triplicate. Absolute recoveries for penillic and penilloic acids were 60-75% depending on the matrix used, whereas corrected recoveries of penicillin G using an isotopically labeled internal standard were ~100%. Two product ion transitions per analyte were required for identification, which contributes to a high degree of selectivity.
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| http://dx.doi.org/10.5740/jaoacint.16-0166 | DOI Listing |
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