A new porcine circovirus-like virus P1 mutant was isolated and sequenced in China in 2015. This P1 mutant has a mutation in its ORF1-encoded capsid protein that results in its elongation by eight amino acids.
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TaqMan-based quantitative real-time polymerase chain reaction assay to detect porcine circovirus-like virus.
Pol J Vet Sci
December 2024
Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, PR China. Email:
The aim of this study was to develop a rapid, sensitive and highly specific TaqMan quantitative real-time polymerase chain reaction PCR (qPCR) assay for porcine circovirus-like virus (PCLV). The primers and probe were designed based on the conserved regions of the PCLV ORF4 gene. The assay has a good detection performance (y=-3.
View Article and Find Full Text PDFLAMP assay coupled with a CRISPR/Cas12a system for the rapid and ultrasensitive detection of porcine circovirus-like virus in the field.
Anal Bioanal Chem
January 2024
Anhui Province Engineering Laboratory for Animal Food Quality and Bio-safety, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
A recent outbreak of porcine circovirus-like virus (PCLV), a virus that may be associated with porcine diarrhea, has been reported in swine herds in China. The virus is spreading rapidly, causing huge economic losses to the swine farming industry. To achieve the rapid, inexpensive, and sensitive detection of PCLV, we combined loop-mediated isothermal amplification (LAMP) and the CRISPR/Cas12a system, whose fluorescence intensity readout can detect PCLV ORF4 gene levels as low as 10 copies.
View Article and Find Full Text PDFVertical transmission of porcine circovirus-like virus P1 in BALB/c mice.
BMC Vet Res
July 2023
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, P. R. China.
Background: Porcine circovirus-like virus P1 is the animal virus with the smallest genome discovered so far, and it has become widely distributed in the Chinese mainland in recent years.
Results: In this study, a BALB/c mouse model was used to reveal P1 infection in female reproductive systems and the vertical transmission of the virus. The female reproductive system, including the ovary and uterus, was harvested on day 14 postinfection and examined for pathological lesions.
Genetic characterization of four strains porcine circovirus-like viruses in pigs with diarrhea in Hunan Province of China.
Front Microbiol
March 2023
Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.
Article Synopsis
- - In this study, researchers discovered a new circular single-stranded DNA virus, named Po-Circo-like (PCL) virus, in intestinal and fecal samples from pigs, potentially linked to diarrhea outbreaks.
- - The PCL virus has a unique genome structure with an ORF1 that codes for a Rep protein, which is crucial for viral replication, differing from similar viruses that typically encode capsid proteins.
- - Four strains of the PCL virus were identified in two pig farms in Hunan Province, China, showing high genetic similarity, indicating it could be a new viral pathogen with implications for cross-species transmission and further studies are recommended to understand its effects.
Detection and genetic analysis of porcine circovirus-like virus in pigs with diarrhea between 2016 and 2021 in Henan and Shanxi provinces of China.
Arch Virol
January 2023
Zhengzhou Major Pig Disease Prevention and Control Laboratory, College of Veterinary Medicine, Henan Agricultural University, Zhengdong New District Longzi Lake 15#, Zhengzhou, 450046, People's Republic of China.
Article Synopsis
- A recently identified virus called porcine circovirus-like virus (PCLV) may be linked to diarrhea in pigs, based on analysis of clinical samples from 175 diseased piglets in China from 2016 to 2021.
- PCLV was found in various sample types with a positive detection rate of 32% overall, higher in certain farms and cities, and often occurred alongside other viral infections, but not with certain common porcine viruses.
- Genetic sequencing of 13 PCLV strains revealed significant similarities to previously documented strains, suggesting PCLV is circulating among pig populations in Henan and Shanxi provinces.
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