https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pubmed&id=27744291&retmode=xml&tool=Litmetric&email=readroberts32@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09 2774429120191120
2046-63905102016Oct15Biology openBiol OpenRefilins are short-lived Actin-bundling proteins that regulate lamellipodium protrusion dynamics.135113611351-136110.1242/bio.019588Refilins (RefilinA and RefilinB) are members of a novel family of Filamin binding proteins that function as molecular switches to conformationally alter the Actin filament network into bundles. We show here that Refilins are extremely labile proteins. An N-terminal PEST/DSG(X)2-4S motif mediates ubiquitin-independent rapid degradation. A second degradation signal is localized within the C-terminus. Only RefilinB is protected from rapid degradation by an auto-inhibitory domain that masks the PEST/DSG(X)2-4S motif. Dual regulation of RefilinA and RefilinB stability was confirmed in rat brain NG2 precursor cells (polydendrocyte). Using loss- and gain-of-function approaches we show that in these cells, and in U373MG cells, Refilins contribute to the dynamics of lamellipodium protrusion by catalysing Actin bundle formation within the lamella Actin network. These studies extend the Actin bundling function of the Refilin-Filamin complex to dynamic regulation of cell membrane remodelling.© 2016. Published by The Company of Biologists Ltd.GayOliviaOINSERM U873 and INSERM Unité 1038, Grenoble F-38000, France CEA, BIG, BGE, Grenoble F-38000, France Université Grenoble Alpes, Grenoble F-38000, France.GilquinBenoîtBINSERM U873 and INSERM Unité 1038, Grenoble F-38000, France CEA, BIG, BGE, Grenoble F-38000, France Université Grenoble Alpes, Grenoble F-38000, France.AssardNicoleNINSERM U873 and INSERM Unité 1038, Grenoble F-38000, France CEA, BIG, BGE, Grenoble F-38000, France Université Grenoble Alpes, Grenoble F-38000, France.StuelsatzPascalPINSERM U873 and INSERM Unité 1038, Grenoble F-38000, France Université Grenoble Alpes, Grenoble F-38000, France.DelphinChristianCINSERM U873 and INSERM Unité 1038, Grenoble F-38000, France Université Grenoble Alpes, Grenoble F-38000, France.LachuerJoëlJGenomic and Microgenomic Platform, ProfileXpert, Bron F-69676, France Lyon Neuroscience Research Center INSERM U1028/CNRS UMR 5292, Lyon F-69372, France.GidrolXavierXINSERM U873 and INSERM Unité 1038, Grenoble F-38000, France CEA, BIG, BGE, Grenoble F-38000, France Université Grenoble Alpes, Grenoble F-38000, France.BaudierJacquesJINSERM U873 and INSERM Unité 1038, Grenoble F-38000, France CEA, BIG, BGE, Grenoble F-38000, France Université Grenoble Alpes, Grenoble F-38000, France jacques.baudier@univ-amu.fr.engJournal Article20161015
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