Micro- and nanoimmunomagnetic beads (MIMBs and NIMBs) used for immunomagnetic separation (IMS) with PCR were studied for the rapid detection of Salmonella. The capture efficiency of the two different IMBs was evaluated by a conventional plate counting method, and the binding pattern was studied using scanning electron microscopy. The specificity of the IMBs was tested with Salmonella, Shigella flexneri, enterohemorrhagic Escherichia coli O157:H7, and Listeria monocytogenes. By comparing the pre-enrichment IMS and the IMS enrichment steps with a 5.5-H enrichment time, this study developed a rapid and sensitive method for the detection of Salmonella in chicken. The method was implemented by IMS enrichment and PCR with MIMBs and NIMBs, with a total analysis time of 8 H. We showed that the method was sensitive based on NIMBs with a detection limit of 10° CFU for Salmonella in 25 g of chicken.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/bab.1539 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Intracellular protein production in bacteria is limited by the need for lysis and costly purification. A promising alternative is to engineer the host organism for protein secretion. While the serovar Typhimurium ( .
View Article and Find Full Text PDFSURVEY OF Salmonella spp., Escherichia coli and Staphylococcus aureus IN ARDEIDS: INVESTIGATION OF POTENTIAL RESERVOIRS.
Microb Pathog
January 2025
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Medicina Veterinária, Universidade Federal de Lavras, UFLA, Campus Universitário, Caixa Postal 3037, CEP 37202-203, Lavras, MG, Brazil. Electronic address:
Ardeids are wild birds well adapted to urban and periurban environments. However, the association of wildlife with humans and livestock increases the chances of transmission of microorganisms between wild animals, domestic animals and humans. The present study aimed to investigate the occurrence of Salmonella spp.
View Article and Find Full Text PDFHigh Prevalence of spp. in Ready-to-Eat Artisanal Pork Sausages Sold at Food Outlets in Quindío, Colombia.
Pathogens
January 2025
Research Group of Microbiology, Industry and Environment (GIMIA), Faculty of Basic Sciences, Universidad Santiago de Cali, Cali 760035, Colombia.
Non-typhoidal salmonellosis is a foodborne disease caused by spp. Most outbreaks of this disease are commonly associated with consuming contaminated meat products, hence the importance of monitoring ready-to-eat artisanal pork sausages for the presence of these bacteria. A total of 494 samples of grilled and smoked barbecue artisanal pork sausages were collected at food outlets from 12 municipalities of the Department of Quindío, Colombia, between 2017 and 2022.
View Article and Find Full Text PDFThe Swab, the Drip, or the Meat? Comparison of Microbiological Sampling Methods in Vacuum-Packed Raw Beef.
Microorganisms
January 2025
UACH-CA03 Tecnología de Alimentos de Origen Animal, Facultad de Zootecnia y Ecología, Universidad Autónoma de Chihuahua, Periférico Fco. R. Almada, Chihuahua 33820, Mexico.
Historically, there has been a concern for the detection and enumeration of microorganisms in foods, and numerous methods have been developed to determine their microbiological conditions. The present study aimed to compare the numbers of microbes recovered with three sampling methods: drip, excision, and swabbing in vacuum-packed beef. The sampling methods were evaluated in terms of the viable numbers of , lactic acid bacteria (LAB), , spp.
View Article and Find Full Text PDFEmerging Resistance and Virulence Patterns in : Insights into Silver Nanoparticles as an Antimicrobial Strategy.
Antibiotics (Basel)
January 2025
The Research Institute of the University of Bucharest (ICUB), 050095 Bucharest, Romania.
Background/objectives: This study aims to characterize antibiotic resistance (AR) and virulence markers in spp. isolated from Romanian outpatients' stool samples.
Methods: In 2019, community-acquired strains were collected and identified using MALDI-TOF mass spectrometry, antibiotic susceptibility profiles have been determined with the MicroScan system, and soluble virulence factors were evaluated using specific culture media, while biofilm formation was quantified in 96-well plates.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!