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The integrative role of cryo electron microscopy in molecular and cellular structural biology. | LitMetric

AI Article Synopsis

  • * New-generation cameras with increased sensitivity are enhancing image recording, leading to better insights into molecular and atomic structures of biological objects.
  • * Cryo-EM is being used alongside techniques like X-ray crystallography and fluorescence imaging, promoting a multi-scale approach that bridges molecular and cellular research, particularly in studying complex structures like ribosomes and viruses.

Article Abstract

After gradually moving away from preparation methods prone to artefacts such as plastic embedding and negative staining for cell sections and single particles, the field of cryo electron microscopy (cryo-EM) is now heading off at unprecedented speed towards high-resolution analysis of biological objects of various sizes. This 'revolution in resolution' is happening largely thanks to new developments of new-generation cameras used for recording the images in the cryo electron microscope which have much increased sensitivity being based on complementary metal oxide semiconductor devices. Combined with advanced image processing and 3D reconstruction, the cryo-EM analysis of nucleoprotein complexes can provide unprecedented insights at molecular and atomic levels and address regulatory mechanisms in the cell. These advances reinforce the integrative role of cryo-EM in synergy with other methods such as X-ray crystallography, fluorescence imaging or focussed-ion beam milling as exemplified here by some recent studies from our laboratory on ribosomes, viruses, chromatin and nuclear receptors. Such multi-scale and multi-resolution approaches allow integrating molecular and cellular levels when applied to purified or in situ macromolecular complexes, thus illustrating the trend of the field towards cellular structural biology.

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Source
http://dx.doi.org/10.1111/boc.201600042DOI Listing

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