AI Article Synopsis
- The study presents a method for immobilizing and stabilizing glucose oxidase using an egg-shell membrane, enabling the development of a simple, reusable glucose sensor.
- The sensor operates effectively across a glucose concentration range of 1 - 1000 mM, with quick response times (70 seconds for high concentrations and 120 seconds for low concentrations) and a limit of detection at 4.761 mM.
- Notably, the sensor can be reused for glucose analysis up to 150 times and maintains effectiveness for over 6 weeks, successfully applied to human blood sample testing.
Article Abstract
In this paper, an egg-shell membrane has been used for efficient immobilization and stabilization of glucose oxidase. This membrane was used for developing a simple and reusable method for estimation of glucose in biological samples. The proposed sensor was effectively used in a wide glucose concentration range (1 - 1000 mM) with fast response time of 70 s for higher concentrations and 120 s for lower concentrations. The results of response study for the fabricated sensor show limit of detection of 4.761 mM with high sensitivity over the entire concentration range (1 - 1000 mM). Most interestingly, the membrane used in the fabricated sensor could be repeatedly used for glucose analysis 150 times and it exhibited a shelf-life of more than 6 weeks. The proposed sensor was also demonstrated for estimation of glucose in human blood samples.
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| http://dx.doi.org/10.2116/analsci.32.1077 | DOI Listing |
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