The development of synthetic chemical probes for the detection of enzymes is extremely important for biological, medicinal, and industrial applications. Here we report the synthesis of an array of novel glycosylated Tb(iii) complexes, their photophysical properties in solution, and their ability to function as luminescent probes for observing glycosidase enzyme activity in real time. Our initial studies into the application of these complexes for the detection of the Concanavalin A (ConA) lectin is also reported, highlighting the broad scope of these novel chemical probes.
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http://dx.doi.org/10.1039/c6ob01712f | DOI Listing |
Mikrochim Acta
January 2025
Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
An exciting upconversion nanoprobe conditioning strategy is proposed to improve the signal-to-background ratio (SBR) through a dye-sensitized strategy, in which the dye functions both as a recognition unit of the detection target and as a sensitizer to amplify the visible luminescence of the lanthanide-doped upconversion nanoparticles (UCNPs), instead of a quencher. The application of this dye-sensitized upconversion nanoprobe to the visual detection of nerve agent mimics diethoxy phosphatidylcholine (DCP) showed excellent detection performance, with up to 110-fold enhancement of the luminescence response of the probe in DCP solution and a detection limit as low as 2 nM. Finally, we performed visual detection of DCP solution and vapor by using test strips containing the probe.
View Article and Find Full Text PDFTalanta
January 2025
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China. Electronic address:
Flavonoid glycosides are formed by dehydration condensation of aglycones and sugar molecules. Therefore, discrimination of flavonoid glycosides from their corresponding aglycones is a challenging task because they contain the same aglycone part in their molecular structures. Herein, boric acid-functional Eu(III)-organic framework (BA-Eu-MOF) was applied to discriminate flavonoid glycosides including baicalin (Bai), wogonoside (Wog), rutin (Rut), puerarin (Pue), quercitrin (Que) and astragalin (Ast) from their corresponding aglycones for the first time.
View Article and Find Full Text PDFInorg Chem
January 2025
Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur, Uttar Pradesh 208016, India.
The nucleotides play multiple fundamental roles that are essential in biochemical enzymatic reactions and signaling pathways. Many diseases are closely associated with their dysregulation. Therefore, reliable and sensitive optical probes to discriminate various nucleotides are essential in biochemistry, drug discovery, and disease diagnosis.
View Article and Find Full Text PDFJ Fluoresc
January 2025
Department of Chemistry, The University of Burdwan, Golapbag, Burdwan, 713104, India.
Nitrogen doped Carbon Quantum Dots (NCQDs) have been synthesized using most economical and easiest hydrothermal process. Here, N-phenyl orthophenylenediamine and citric acid were utilised as a source of nitrogen and carbon for the preparation of NCQDs. The synthesized NCQDs were characterized using experimental techniques like UV - Vis absorption, FT-IR, transmission electron microscopy (TEM), X-ray Diffraction (XRD), EDX, dynamic light scattering (DLS), fluorimeter and time resolved fluorescence spectroscopy.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
January 2025
Zhejiang Cancer Hospital, Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou 310022, PR China; School of Molecular Medicine, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, PR China.
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