We prepared 0.3 or 0.15mm i.d. columns from both fused silica capillaries and planar titanium wafers with machined grooves. Both types of devices were packed with sub-two micron C18 sorbent. Chromatographic efficiency and peak capacity were tested using LC instruments with low extra column dispersion (300nL or 30nL for 0.3 or 0.15mm i.d. columns, respectively). Micro column testing in gradient mode was less affected by extra column (pre-column) dispersion. To exploit this feature we developed a method for estimation of column efficiency from gradient analysis using the theoretical relationship (P-1)=N×const. The validity of this relationship was experimentally verified using 2.1mm i.d. and 0.3mm i.d. columns. The (P-1) versus N relationship was experimentally determined with straight columns, which in turn was employed for the estimation of microfluidic column efficiency. Microfluidic devices with serpentine channels exhibited lower isocratic efficiency than straight capillary columns, but the loss of peak capacity was less significant. The loss chromatographic efficiency due to zone dispersion in serpentine microfluidic channels was more apparent for 0.3 than 0.15mm i.d. devices. Gradient performance of 0.15×100mm microfluidic columns was comparable to state-of-the-art 2.1×100mm columns packed with the same sorbent.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.chroma.2016.10.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A hybrid encryption framework leveraging quantum and classical cryptography for secure transmission of medical images in IoT-based telemedicine networks.
Sci Rep
December 2024
School of Electronic and Nanoscale Engineering, University of Glasgow, Glasgow, G12 8QQ, UK.
In the era of the Internet of Things (IoT), the transmission of medical reports in the form of scan images for collaborative diagnosis is vital for any telemedicine network. In this context, ensuring secure transmission and communication is necessary to protect medical data to maintain privacy. To address such privacy concerns and secure medical images against cyberattacks, this research presents a robust hybrid encryption framework that integrates quantum, and classical cryptographic methods.
View Article and Find Full Text PDFComparing CT-like bone images based on FRACTURE MR with CT in pediatric congenital vertebral anomalies.
AJNR Am J Neuroradiol
December 2024
From the Department of Radiology (H.N.M., F.B.G.), Bai Jerbai Wadia Hospital for Children, Mumbai, Maharashtra, India.
Background And Purpose: Congenital vertebral anomalies are commonly associated with underlying spinal cord anomaly which necessitates imaging both the spinal cord and the bony vertebral column to understand the extent of the deformity better. While MRI is the gold standard for spinal cord imaging, it does not provide CT-like bone details. Many MR bone imaging techniques have been tested in various adult spine conditions in the past decade but not much has been described on their reliability in pediatric spine.
View Article and Find Full Text PDFEfficient nitrate removal via microorganism-iron oxide co-evolution on biocathode surface.
Bioelectrochemistry
December 2024
School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353 Shandong, PR China; State Key Laboratory of Bio-based Materials and Green Papermaking, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, Shandong, PR China. Electronic address:
Sediment microbial fuel cell (SMFC) is a device for biological denitrification, in which electrons produced by sediment microorganisms can be transferred to the upper layer of the water column lacking electron donors. However, the low efficiency of denitrifying bacteria in acquiring electrons and enriching at the cathode greatly hinders the application of SMFC for nitrogen removal. In this study, we report a novel method of constructing a high-performance biocathode by modifying electrodes with zero-valent iron to enhance the enrichment and electron transfer of electroactive bacteria.
View Article and Find Full Text PDFN-functionalized calcium alginate encapsulated with microalgae for efficient Pb removal: Mechanisms and performance in batch and column operations.
J Hazard Mater
December 2024
Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea. Electronic address:
In this study, a novel adsorbent called Ca@SP was developed by immobilizing microalgae protein (Spirulina platensis, SP) in an alginate matrix for enhanced Pb²⁺ removal from aqueous solutions. Synthesized via in situ crosslinking, Ca@SP leverages the synergistic effects of alginate's gel-forming ability and SP's N-rich biomass. Characterization of Ca@SP revealed a green spherical hydrogel with a BET specific surface area of 159.
View Article and Find Full Text PDFMaintaining stability at the lumbosacral-pelvic region in adult spinal deformity surgery without sacroiliac joint fusion: are 4 pelvic screws superior to 2 pelvic screws?
J Neurosurg Spine
December 2024
1Department of Orthopaedic Surgery, The Och Spine Hospital/Columbia University Irving Medical Center, New York, New York.
Objective: The objective of this study was to compare a multiple pelvic screw fixation strategy (dual bilateral 4 pelvic screw fixation [4PvS]) with the use of single bilateral 2 pelvic screw fixation (2PvS), with the aim of addressing lumbosacral junction stability.
Methods: This analysis is a single-center, retrospective review of ASD patients treated between 2015 and 2021. All patients had a minimum 2-year follow-up and spinal fusion to the sacrum without sacroiliac fusion and met at least one radiographic and procedural criterion: pelvic incidence-lumbar lordosis ≥ 20°, T1 pelvic angle ≥ 20°, sagittal vertical axis ≥ 7.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!