AI Article Synopsis
- Glioma is the most frequent primary brain tumor, and this study investigates the roles of miR-101 and COX-2 in its development.
- The research found that miR-101 levels were lower, while COX-2 levels were higher in glioma cells compared to normal cells, suggesting a link between these molecules.
- When miR-101 was introduced into glioma cells, it reduced COX-2 levels and inhibited cell growth and migration, indicating that miR-101 downregulation contributes to glioma progression through COX-2 upregulation.
Article Abstract
Glioma is the most common type of primary tumor of the central nervous system. The present study aimed to demonstrate the role of miR-101 and cyclooxygenase-2 (COX-2) in the initiation and development of glioma. The expression of miR-101 and COX-2 in normal and malignant human glial cells and tissues was determined by western blotting and quantitative polymerase chain reaction analysis. The role of miR-101 on COX-2 expression was evaluated by a dual-luciferase reporter assay. The effects of miR-101 and COX-2 in glioma cell proliferation and invasion was verified by CCK-8 test and Transwell assays, respectively. The present study demonstrated that miR-101 expression was downregulated while COX-2 was upregulated in glioma tissues and cells. Furthermore, transfection of miR-101 significantly downregulated COX-2 expression in both U373 and U87 glioma cells. In addition, further experiments revealed that overexpression of miR-101 resulted in significant inhibition of the proliferation and migration of glioma cells, and the growth of established tumors. Direct downregulation of COX-2 by transfection with corresponding small interfering RNA also inhibited the proliferation and invasion of glioma cells. These results indicate that downregulation of miR-101 is involved in the initiation and development of glioma via COX-2 upregulation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5038506 | PMC |
| http://dx.doi.org/10.3892/ol.2016.4939 | DOI Listing |
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