Bioactivity of Diterpens from the Ethyl Acetate Extract of Rox. Hams.

Background: Rox. Hams. is an endangered medicinal plant used in gonorrhoe, catarrhal conditions of genito-urinary and respiratory tracts. The scientific and pharmacological formulation of has not been established so far though it is being traditionally used by tribes of the region.

Objective: P hytochemical screening and identification of the bioactive compounds from the ethyl acetate extract of Rox. Hams.

Materials And Methods: Chromatographic separation was carried out by thin layer chromatography and column chromatography. Bio-autography of the column fractioned extract and TLC chromatogram were evaluated for antibacterial activity. The PTLC, HP TLC were used for crude extract and HPLC, LCMS, FTIR, HNMR and CNMR were employed for the isolated compound in the ethyl acetate extract of .

Results: Evaluation of solvent system for chromatographic separation revealed that ethyl acetate: petroleum ether in the ratio of 7:2.5 ml was the most appropriate one for the separation of diterpene compounds. The antibacterial bio-autography screening of TLC separated compound showed positive activity with and negative activity with . Spectroscopic analysis of the isolated compound from the ethyl acetate extract of revealed the presence of diterpene compound.

Conclusion: It is evident from the present study that the ethyl acetate extract of is rich in diterpene compounds and having potential antibacterial activity.

Summary: Novel extraction method for phytochemicls from Kingidendron pinnatum at RTAntibacterial property of diterpens extracted from Rox. Hams aganist S. aureus : TLC: Thin Layer Chromatography, PTLC: Preparatory Thin Layer Chromatography, HPTLC: High perormence Thin Layer chromatography, HPLC: High Performance Liquid Chromatography, LC-MS: Liquid chromatography Mass Spectra, FTIR: Fourier Transform Infrared Chromatography, NMR: Nuclear Magnetic Resonance.