Two-Dimensional Visualization of Nitrogen Distribution in Leaves of Chinese Cabbage (Brassica rapa subsp. chinensis) by the Fourier Transform Infrared Photoacoustic Spectroscopy Technique.

Understanding nitrogen (N) status in the leaves of Chinese cabbage (Brassica rapa subsp. chinensis) is of significance to both vegetable growth and quality control. Fourier transform infrared photoacoustic spectroscopy was used to perform rapid qualification of N distribution in leaves; a partial least squares algorithm was used to develop a model for prediction of the N content; and N distribution in individual leaves was mapped on the basis of interpolation analysis, which was found to be variable. A reasonable N input level (13 mmol L N) showed the largest variance of the N content, benefiting N redistribution and use efficiency, but variance decreased at the old stage. Moreover, the pattern of N distribution within a leaf was irregular even among the replications performed for each treatment, and sunlight was found to be the dominant factor as a result of leaves receiving variable light intensities.