AI Article Synopsis
- The MEP pathway is essential for producing important metabolites like carotenoids and tocopherols in plants, with a focus on sweet osmanthus.
- Ten MEP pathway genes were identified in sweet osmanthus and showed high similarity to genes in other species, indicating conserved functions.
- These genes exhibited unique expression patterns in different plant tissues and at various times, with significant expression in inflorescences, highlighting their potential roles in plant development and metabolism.
Article Abstract
The 2--methyl-d-erythritol 4-phosphate (MEP) pathway is responsible for the biosynthesis of many crucial secondary metabolites, such as carotenoids, monoterpenes, plastoquinone, and tocopherols. In this study, we isolated and identified 10 MEP pathway genes in the important aromatic plant sweet osmanthus (). Multiple sequence alignments revealed that 10 MEP pathway genes shared high identities with other reported proteins. The genes showed distinctive expression profiles in various tissues, or at different flower stages and diel time points. The qRT-PCR results demonstrated that these genes were highly expressed in inflorescences, which suggested a tissue-specific transcript pattern. Our results also showed that , , and had a clear diurnal oscillation pattern. The isolation and expression analysis provides a strong foundation for further research on the MEP pathway involved in gene function and molecular evolution, and improves our understanding of the molecular mechanism underlying this pathway in plants.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5083917 | PMC |
| http://dx.doi.org/10.3390/genes7100078 | DOI Listing |
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