Peripheral blood mononuclear cell samples from human immunodeficiency virus (HIV) antibody-positive subjects were inoculated into cell cultures used for routine viral isolation to determine if HIV could survive in them. HIV was recovered from 5 (19%) of 26 day 1 supernatants and from four of the five samples in all three types of cell cultures. We conclude that HIV may survive for at least 24 h in standard virology cell culture systems and therefore is a potential risk for laboratory personnel.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC267637PMC
http://dx.doi.org/10.1128/jcm.27.7.1664-1666.1989DOI Listing

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