AI Article Synopsis
- The study aimed to assess porcine sperm vitrification using cryoloops with and without two cryoprotectants, and to evaluate two warming procedures.
- The research involved diluting extended and raw semen with different media, vitrifying at a concentration of 20 million spermatozoa per ml, and examining sperm viability, motility, and chromatin properties before and after vitrification.
- Results showed that after vitrification, chromatin condensation and integrity were preserved across all conditions, indicating potential for future applications, like producing porcine embryos via intracytoplasmic sperm injection using vitrified sperm.
Article Abstract
The aims of this study were to evaluate porcine sperm vitrification in cryoloops, with and without two different cryoprotectants and assess two warming procedures. Extended (n = 3; r = 4) and raw (n = 5; r = 2) semen was diluted in media without and with cryoprotectants (4% dimethylformamide and 4% glycerol) to a final concentration of 20 × 10 spermatozoa ml and vitrified using the cryoloops method. Two warming procedures were evaluated: rapid method (30 s at 37°C) and an ultra-rapid method (7 s at 75°C, followed by 30 s at 37°C). Total motility (phase contrast), sperm viability (6-carboxifluorescein diacetate and propidium iodide stain), membrane function (hypo-osmotic swelling test), acrosome integrity (phase contrast), chromatin condensation (toluidine blue stain) and chromatin susceptibility to acid denaturation (acridine orange stain) were evaluated before and after vitrification and analysed using Friedman's test. In all media, the only seminal parameters that were maintained after vitrification were chromatin condensation and integrity. Vitrification of porcine spermatozoon using cryoloops, both in the presence or absence of cryoprotectants and independent of the warming procedure used, permits conservation of sperm chromatin condensation and integrity. It would be interesting to further verify this by producing porcine embryos using vitrified spermatozoon with intracytoplasmic sperm injection.
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| http://dx.doi.org/10.1111/and.12706 | DOI Listing |
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