The application of ultrasound to a solution can induce cavitional phenomena and generate high localised temperatures and pressures. These are dependent of the frequency used and have enabled ultrasound application in areas such as synthetic, green and food chemistry. High frequency (100kHz to 1MHz) in particular is promising in food chemistry as a means to inactivate enzymes, replacing the need to use periods of high temperature. A plant enzyme, horseradish peroxidase, was studied using time-resolved fluorescence techniques as a means to assess the effect of high frequency (378kHz and 583kHz) ultrasound treatment at equivalent acoustic powers. This uncovered the fluorescence emission from a newly formed species, attributed to the formation of di-tyrosine within the horseradish peroxidase structure caused by auto-oxidation, and linked to enzyme inactivation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.saa.2016.09.035 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Shadow Effect-Triggered Photosensitive Gate of Organic Photoelectrochemical Transistor for Enhanced Biodetection.
Anal Chem
December 2024
School of Materials, Shenzhen Campus of Sun Yat-sen University, Shenzhen, Guangdong 518107, P. R. China.
The integration of a photosensitive gate into an organic electrochemical transistor has currently emerged as a promising route for biological sensing. However, the modification of the photosensitive gate always involves complex processes, and the degradation of sensitivity of the functional materials under illumination will significantly decrease the stability of the devices. Herein, we designed an organic photoelectrochemical transistor (OPECT) biosensor employing horseradish peroxidase (HRP)@glucose oxidase (GOx)/Pt/n-Si as the photosensitive gate based on the "shadow effect".
View Article and Find Full Text PDFNon-antibiotic dependent photothermal antibacterial hemostatic MXene hydrogel for infectious wounds healing.
Biomater Adv
December 2024
National and Local Joint Engineering Laboratory for Synthetic Technology of High Performance Polymer, College of Chemistry, Jilin University, Changchun 130012, China. Electronic address:
On account of the existence of antibiotic resistance, the wound healing of pathogenic infection is still a challenge in modern society. A desirable wound dressing should own the abilities of adhesiveness, hemostasis and good mechanical property, meanwhile the property of eliminating bacteria without side effects is also highly needed. In this work, we established a kind of hydrogel based on carboxymethyl cellulose-graft-tyramine (CMC-Ty) and MXene (TiCT) through employing HO/HRP (horseradish peroxidase) as the initiator, then the as-prepared hydrogel (named CMC-Ty/MXene) was immersed in tannic acid (TA) solution, and this TA-treated hydrogel was called CMC-Ty/MXene+TA.
View Article and Find Full Text PDFDual Activities of Flower-like Gold-Iron Oxide Nanozyme for Peroxidase-mimicking and Glucose Detection.
Chem Asian J
December 2024
Indian Institute of Technology Roorkee, Department of Chemistry, Indian Institute of Technology Roorkee, 247667, Roorkee, INDIA.
Nanozymes, constituting of inorganic nanomaterials, are the sustainable and cost-effective alternatives of the naturally abundant enzymes. For more than a decade, nanozymes have shown astonishingly enhanced enzymatic activity as compared to its naturally occurring counterpart and emerged as a potential platform in biomedical science. The current study reports a novel flower shaped gold-iron oxide nanocomposite prepared via a facile and green solution phase redox mediated synthesis.
View Article and Find Full Text PDFHybrid Enzyme-Electrocatalyst Cascade Modified Gas-diffusion Electrodes for Methanol Formation from Carbon Dioxide.
Angew Chem Int Ed Engl
December 2024
Ruhr University Bochum, Analytische Chemie, Universitätsstr 150, 44780, Bochum, GERMANY.
We propose a hybrid electrocatalytic-bioelectrocatalytic reaction cascade integrated on a gas diffusion electrode for CO2 reduction under selective formation of methanol. Ag-Bi2O3 selectively reduces gaseous CO2 to formate at neutral pH conditions. A subsequent enzymatic cascade comprising formaldehyde dehydro-genase and alcohol dehydrogenase, which are both nicotinamide adenine dinucleotide (NAD)-dependent, further reduce formate sequentially to formaldehyde and methanol.
View Article and Find Full Text PDFVanadium single-atoms coordinated artificial peroxidases as biocatalyst-linked immunosorbent assay for highly-sensitive carcinoembryonic antigen immunoassay.
Biomaterials
December 2024
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, China; Department of Endodontics, Department of Orthodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China. Electronic address:
In medical and biomedical fields, enzyme-mimetic nanomaterials have garnered significant interest as efficacious signal enhancers for biocatalyst-linked immunosorbent assays (BLISA). Despite the burgeoning enthusiasm, engineering artificial biocatalysts that exhibit both exceptional catalytic proficiency and pronounced colorimetric signal output remains a formidable challenge. Inspired by the heme structures and biocatalytic activities of horseradish peroxidase, we introduce the synthesis of vanadium single-atoms (SAV) coordinated artificial peroxidases as BLISA for highly sensitive and selective carcinoembryonic antigen (CEA) immunoassay.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!