The present research work was undertaken with a mandate to study carbon spectrum utilization and structural characterization of biosurfactant produced by indigenous Pseudomonas aeruginosa NCIM 5514, which showed unique properties to utilize a large number of carbon sources effectively for production of biosurfactant, although glucose was the best carbon substrate. In Bushnell-Hass medium supplemented with glucose (1%, w/v), 3.178±0.071g/l biosurfactant was produced by this isolate in 96h. The biosurfactant produced showed surface tension and emulsification activity values from 29.14±0.05 to 62.29±0.13mN/m and 88.50±1.96 to 15.40±0.91%, respectively. Toluene showed highest emulsification activity followed by kerosene. However, kerosene exhibited emulsion stability for 30days. Biosurfactant was characterized as a mixture of di-rhamnolipid (Rha-Rha-C-C) and mono-rhamnolipid (Rha-C-C) by FTIR, ESI-MS and LC-MS techniques. High biosurfactant yield opens up doors for the isolate to find utility in various industries.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.biortech.2016.09.080 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Application of biomanufacturing in polymer flooding].
Sheng Wu Gong Cheng Xue Bao
January 2025
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
In China, the crude oil supply is highly dependent on overseas countries, and thus strengthening crude oil self-sufficiency has become an important issue of the national energy security. Tertiary oil recovery, especially polymer flooding, has been widely applied in large oil fields in China, which can increase the recovery rate by 15%-20% compared with water flooding. However, the widely used oil flooding polymers show poor thermal stability and salinity tolerance, complicated synthesis ways of monomers, and environmental unfriendliness.
View Article and Find Full Text PDFSurfactin accelerates pellicle biofilm development.
Biofilm
June 2025
DTU Bioengineering, Technical University of Denmark, 2800, Kongens Lyngby, Denmark.
Surfactin is a biosurfactant produced by many strains with a wide variety of functions from lowering surface tension to allowing motility of bacterial swarms, acting as a signaling molecule, and even exhibiting antimicrobial activities. However, the impact of surfactin during biofilm formation has been debated with variable findings between studies depending on the experimental conditions. B.
View Article and Find Full Text PDFNew Insights on Strain 1B Surface-Active Biomolecules: Gordofactin Properties.
Molecules
December 2024
Unidade de Bioenergia e Biorrefinarias, LNEG-Laboratório Nacional de Energia e Geologia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
Biosurfactants/bioemulsifiers (BSs/BEs) can be defined as surface-active biomolecules produced by microorganisms with a broad range of applications. In recent years, due to their unique properties like biodegradability, specificity, low toxicity, and relative ease of preparation, these biomolecules have attracted wide interest as an eco-friendly alternative for several industrial sectors, escalating global microbial BS/BE market growth. Recently, strain 1B, a bacterium with significant biotechnological potential, well known for its biodesulfurizing properties, carotenoid production, and broad catabolic range, was described as a BS/BE producer.
View Article and Find Full Text PDFBioprocess development for microbial production and purification of cellobiose lipids by the smut fungus Ustilago maydis DSM 4500.
Bioprocess Biosyst Eng
January 2025
Department of Chemical Engineering, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
Cellobiose lipids (CBLs) are a class of glycolipid biosurfactants produced by various fungal strains. These compounds have gained significant interest due to their surface-active and antifungal properties, which are comparable to traditional synthetic surfactants and antimicrobials. Despite their potential applicability in various cosmetic, pharmaceutical, and agricultural formulations, significantly less research has been focused on their production and purification in comparison to other glycolipid biosurfactants, such as mannosylerythritol lipids (MELs) and sophorolipids.
View Article and Find Full Text PDFExploring Novel Fungal-Bacterial Consortia for Enhanced Petroleum Hydrocarbon Degradation.
Toxics
December 2024
Postgraduate Program in Biotechnology and Biosciences, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina-Campus Reitor João David Ferreira Lima, Florianópolis 88040-900, SC, Brazil.
Bioremediation, involving the strategic use of microorganisms, has proven to be a cost-effective alternative for restoring areas impacted by persistent contaminants such as polycyclic aromatic hydrocarbons (PAHs). In this context, the aim of this study was to explore hydrocarbon-degrading microbial consortia by prospecting native species from soils contaminated with blends of diesel and biodiesel (20% biodiesel/80% diesel). After enrichment in a minimal medium containing diesel oil as the sole carbon source and based on 16S rRNA, Calmodulin and β-tubulin gene sequencing, seven fungi and 12 bacteria were identified.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!