is a common opportunistic pathogen known for its ability to colonize healthy piglets and causes Glässer's disease. The lipooligosaccharide (LOS) of is a potential virulence-associated factor. In this study, two putative glycosyltransferases that might be involved in LOS synthesis in SC096 were identified ( and ). Mutants were constructed to investigate the roles of the and genes. The LOS from the Δ or Δ mutant showed truncated structure on silver-stained SDS-PAGE gel compared to the wild-type strain. The Δ and Δ mutants were significantly more sensitive to 50% porcine serum, displaying 15.0 and 54.46% survival rates, respectively. Complementation of the mutant restored the serum-resistant phenotype. Additionally, the Δ and Δ strains showed impaired ability to adhere to and invade porcine kidney epithelial cells (PK-15). The above results suggested that the and genes of the SC096 strain participated in LOS synthesis and were involved in serum resistance, adhesion and invasion.
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| http://dx.doi.org/10.3389/fcimb.2016.00100 | DOI Listing |
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