Male infertility and the poor quality of sperm seem to be influenced by oxidative stress. In particular, the reactive oxygen species (ROS) mainly produced by morphologically altered spermatozoa affect sperm motility, morphology, and integrity. The aim of this study was to evaluate the efficacy of Myo-Inositol (Myo-Ins) on a number of parameters such as viscosity and total and progressive motility of spermatozoa, in order to better validate its possible practical application in vitro, in order to improve the capacitation protocols commonly used in Assisted Reproductive Technology (ART). A total of 100 fresh and 25 thawed semen samples were analyzed in vitro prior to and after addition of Myo-Ins. Treatment of samples with Myo-Ins showed an increase in the sperm total and progressive motility in both fresh and thawed samples. Furthermore, Myo-Ins proved to be well tolerated by spermatozoa in vitro, demonstrating that it can be efficiently and safely used as antioxidant in the laboratory practice and for preparation of semen samples in ART.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031851 | PMC |
| http://dx.doi.org/10.1155/2016/2839041 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Computer Vision-Based Fire-Ice Ion Algorithm for Rapid and Nondestructive Authentication of Ziziphi Spinosae Semen and Its Counterfeits.
Foods
December 2024
Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
The authentication of Ziziphi Spinosae Semen (ZSS), Ziziphi Mauritianae Semen (ZMS), and Hovenia Acerba Semen (HAS) has become challenging. The chromatic and textural properties of ZSS, ZMS, and HAS are analyzed in this study. Color features were extracted via RGB, CIELAB, and HSI spaces, whereas texture information was analyzed via the gray-level co-occurrence matrix (GLCM) and Law's texture feature analysis.
View Article and Find Full Text PDFMelatonin in Male Dromedary Camel () Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes.
Animals (Basel)
January 2025
Livestock and Wildlife Laboratory (LR16IRA04), Arid Lands Institute, University of Gabès, Médenine 4119, Tunisia.
Camels () are seasonal short-day breeders, regulated by photoperiod and melatonin secretion. However, no studies have explored melatonin levels in camel seminal plasma or their relationship with testosterone, age, or climatic factors, nor is it known whether melatonin receptors exist in camel spermatozoa to respond to seminal melatonin. This study aimed to analyze melatonin levels in camel seminal plasma and its specific receptors in spermatozoa.
View Article and Find Full Text PDFThe Effect of Cholesterol-Loaded Cyclodextrin and Resveratrol Compounds on Post-Thawing Quality of Ram Semen.
Vet Med Sci
January 2025
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, İstanbul University-Cerrahpasa, Avcilar, İstanbul, Turkey.
Ram sperm are more vulnerable to freezing than those of most other farm animals. During sperm freezing, the cell membrane loses some of its cholesterol, which regulates signalling mechanisms and prevents premature capacitation. Resveratrol (RES) increases the fluidity of the cell membrane, which becomes peroxidized during freezing and reduces free radicals.
View Article and Find Full Text PDFFluoxetine Mitigates Human Sperm Quality by Disrupting the Antioxidant Defense System and Altering the Expression of Apoptosis-Related Genes: An In Vitro Study.
Reprod Sci
January 2025
Department of Biology, Faculty of Science, University of Qom, Qom, 3716146611, Iran.
Fluoxetine is used in the management of depression, anxiety and other mood disorders by increasing serotonin levels in the brain and can cause sexual side effects by changing the homeostasis of sex hormones and increasing oxidative stress. Since many men who take fluoxetine are of reproductive age and sperm are exposed to fluoxetine for a considerable time, this study aimed to examine the in vitro effects of fluoxetine on human sperm biochemical markers and sperm parameters. Semen samples from 30 fertile men were divided into three groups: a positive control group, a negative control group and a fluoxetine-treated group.
View Article and Find Full Text PDFValidation of a mail-in delayed semen analysis protocol developed for home collection.
F S Rep
December 2024
Quest Diagnostics, Secaucus, New Jersey.
Objective: To validate a mail-in delayed semen analysis service using deidentified remnant samples from a US fertility clinic.
Design: Double-blinded prospective validation of screening/diagnostic test.
Setting: Fertility clinic and clinical reference laboratory.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!