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Molecular Identification and Traceability of Illegal Trading in (Teleostei: Characiformes), a Threatened Brazilian Fish Species, Using DNA Barcode. | LitMetric
is a threatened freshwater fish species and endemic of a few coastal rivers in northeastern Brazil. Even though the Brazilian laws prohibit the fisheries of threatened species, is occasionally found in street markets, being highly appreciated by local population. In order to provide a reliable DNA barcode dataset for , we compared mitochondrial sequences of cytochrome c oxidase subunit I (COI) from fresh, frozen, and salt-preserved specimens. Phylogenetically related species ( spp.) and other fish species () commonly mixed with in street markets were also included to test the efficiency of molecular identification. In spite of the differences in conservation processes and advanced deterioration of some commercial samples, high-quality COI sequences were obtained and effective in discriminating specimens. In addition, while populations from Contas and Almada River basins seem to comprise a single evolutionary lineage, the specimens from Cachoeira River were genetically differentiated, indicating population structuring. Therefore, DNA barcoding has proved to be useful to trace the illegal trading of and to manage threatened populations, which should focus on conservation of distinct genetic stocks and mitigation on human impacts along their range.
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