AI Article Synopsis
- This chapter discusses the study of Stress-Inducible Protein 1 (STI1) found in extracellular vesicles.
- The researchers employed various techniques like ultracentrifugation and gel chromatography to isolate the vesicles.
- They used nanotracking particle analysis, flow cytometry, and ELISA to analyze the size, number, and protein content of these vesicles.
Article Abstract
This chapter is derived from our experience in the study of stress-Inducible Protein 1 (STI1) in extracellular vesicles. We used different techniques to isolate, explore, and characterize the extracellular vesicles that contained this protein. Ultracentrifugation and gel chromatography were used to isolate extracellular vesicles of different sizes, nanotracking particle analysis (NTA) determined number and size of vesicles, while flow cytometry and ELISA were used to determine the specific protein content of vesicles.
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| http://dx.doi.org/10.1007/978-1-4939-3804-9_11 | DOI Listing |
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